M-Pseudomonas Broth

Pseudomonas aeruginosa is characterized by the production of pyocyanin (a blue green, water
soluble, non-fluorescent, phenazine pigment) which is stimulated by the inclusion of magnesium
chloride and potassium sulfate in the Pseudomonas Isolation Broth. Irgasan, an antimicrobial agent,
selectively inhibits gram-positive and gram-negative bacteria other than pseudomonads. Glycerol
both serves as an energy source and helps in the promotion of pyocyanin.