Western Blotting

Western blotting is a common and important technique used in molecular biology. It is used to detect a specific protein or protein
fragment from a complex mixture such as a cell lysate, tissue extract, blood or serum sample or culture supernatants. The complex mixture is separated according to size by gel electrophoresis and then transferred to a membrane. A protein of specific interest is
immunodetected using primary and secondary antibodies.

Pure Nitrocellulose

Nitrocellulose Pure Transfer Membrane is the membrane of
choice for all protein or immunoblotting applications. The high
sensitivity of GVS Nitrocellulose Transfer Membrane ensures
excellent results in all transfers, especially in protein blotting.
Unlike PVDF, nitrocellulose wets out naturally, does not
require methanol, and will not turn hydrophobic during the
transfer process.
Nitrocellulose is very easily blocked and does not need the many
blocking steps required with PVDF.
Excellent results will be obtained with all detection
systems: antibody/antigen, radiolabeled, biotinylated, and
chemiluminescent, giving you a great amount of flexibility in
designing your procedure.

Polyvinylidene Fluoride PVDF

PVDF is a naturally hydrophobic, unsupported transfer
membrane. It has a high binding capacity, which prevents
protein from passing through the membrane, and a low
background that provides for an excellent signal-noise ratio.
It also has exceptional tensile strength, preventing it from
cracking, tearing, breaking or curling. This membrane also has
broad chemical compatibility, which is important when used
with common stains such as Amido Black, Colloidal Gold,
Coomassie Blue, India Ink and Ponceau-S. GVS PVDF will
not degrade, distort or shrink when a high concentration of
methanol is used for destaining.

Supported Nitrocellulose MCE

GVS Supported NitrocelluloseTransfer Membrane combines
the binding characteristics of nitrocellulose membrane with
the strength of nylon membrane. It can be easily used in any
protocol utilizing unsupported nitrocellulose transfer membrane.
Supplied in various porosity and format