Western Blotting

Western blotting is a common and important technique used in molecular biology. It is used to detect a specific protein or protein
fragment from a complex mixture such as a cell lysate, tissue extract, blood or serum sample or culture supernatants. The complex mixture is separated according to size by gel electrophoresis and then transferred to a membrane. A protein of specific interest is
immunodetected using primary and secondary antibodies.

Pure Nitrocellulose

Nitrocellulose Pure Transfer Membrane is the membrane of
choice for all protein or immunoblotting applications. The high
sensitivity of GVS Nitrocellulose Transfer Membrane ensures
excellent results in all transfers, especially in protein blotting.
Unlike PVDF, nitrocellulose wets out naturally, does not
require methanol, and will not turn hydrophobic during the
transfer process.
Nitrocellulose is very easily blocked and does not need the many
blocking steps required with PVDF.
Excellent results will be obtained with all detection
systems: antibody/antigen, radiolabeled, biotinylated, and
chemiluminescent, giving you a great amount of flexibility in
designing your procedure.

SKUDescriptionPore SizeTypePriceBuy
31L1020

Transfer Membrane 300x500mm NC Unsupported

0.22umSheet$47.00 10/pack
31L1021

Transfer Membrane 100x100mm NC Unsupported

0.22umSheet$104.00 10/pack
31L1023

Transfer Membrane 200x200mm NC Unsupported

0.22umSheet$521.00 25/pack
31L1024

Transfer Membrane 300x300mm NC Unsupported

0.22umRoll$359.00 1/pack
31L1029

Transfer Membrane 200x300mm NC Unsupported

0.22umRoll$242.00 1/pack
31L1031

Transfer Membrane 300x300mm NC Unsupported

0.45umRoll$325.00 1/pack
31L1035

Transfer Membrane 150x150mm NC Unsupported

0.45umSheet$76.00 5/pack
31L1041

Transfer Membrane 200x300mm NC Unsupported

0.45umRoll$218.00 1/pack
31L1043

Transfer Membrane 200x200mm NC Unsupported

0.45umSheet$478.00 25/pack

Polyvinylidene Fluoride PVDF

PVDF is a naturally hydrophobic, unsupported transfer
membrane. It has a high binding capacity, which prevents
protein from passing through the membrane, and a low
background that provides for an excellent signal-noise ratio.
It also has exceptional tensile strength, preventing it from
cracking, tearing, breaking or curling. This membrane also has
broad chemical compatibility, which is important when used
with common stains such as Amido Black, Colloidal Gold,
Coomassie Blue, India Ink and Ponceau-S. GVS PVDF will
not degrade, distort or shrink when a high concentration of
methanol is used for destaining.

SKUDescriptionPore SizeTypePriceBuy
31L1045

Transfer Membrane 150x150mm PVDF

0.45umSheet$109.00 5/pack
31L1046

Transfer Membrane 200x200mm PVDF

0.45umSheet$156.00 5/pack
31L1047

Transfer Membrane 300x300mm PVDF

0.45umRoll$370.00 1/pack
31L1049

Transfer Membrane 100x100mm PVDF

0.45umSheet$139.00 10/pack
31L1051

Transfer Membrane 200x300mm PVDF

0.45umRoll$265.00 1/pack
31L1053

Transfer Membrane 300x300mm PVDF

0.22umRoll$370.00 1/pack
31L1055

Transfer Membrane 70x84mm PVDF

0.22umSheet$110.00 10/pack
31L1056

Transfer Membrane 200x300mm PVDF

0.22umRoll$265.00 1/pack
31L1059

Transfer Membrane 200x200mm PVDF

0.22umSheet$156.00 5/pack

Supported Nitrocellulose MCE

GVS Supported NitrocelluloseTransfer Membrane combines
the binding characteristics of nitrocellulose membrane with
the strength of nylon membrane. It can be easily used in any
protocol utilizing unsupported nitrocellulose transfer membrane.
Supplied in various porosity and format

SKUDescriptionPore SizeTypePriceBuy
31L1069

Transfer Membrane 300x300mm  NC Supported (MCE)

0.45umRoll$328.00 1/pack
31L1073

Transfer Membrane 150x150mm  NC Supported (MCE)

0.45umSheet$77.00 5/pack
31L1074

Transfer Membrane 200x200mm  NC Supported (MCE)

0.45umSheet$131.00 5/pack
31L1078

Transfer Membrane 200x300mm  NC Supported (MCE)

0.45umRoll$223.00 1/pack
31L1080

Transfer Membrane 300x300mm  NC Supported (MCE)

0.22umRoll$362.00 1/pack
31L1081

Transfer Membrane 82mm  NC Supported (MCE)

0.22umDisc$160.00 50/pack
31L1082

Transfer Membrane 150x150mm  NC Supported (MCE)

0.22umSheet$81.00 5/pack
31L1083

Transfer Membrane 200x200mm  NC Supported (MCE)

0.22umSheet$142.00 5/pack
31L1084

Transfer Membrane 200x300mm  NC Supported (MCE)

0.22umRoll$244.00 1/pack
31L1089

Transfer Membrane 100x100mm  NC Supported (MCE)

0.22umSheet$104.00 10/pack
31L1092

Transfer Membrane 70x84mm  NC Supported (MCE)

0.45umSheet$75.00 10/pack